A vaccine strategy that protects against genital herpes by establishing local memory T cells.

Most successful existing vaccines rely on neutralizing antibodies, which may not require specific anatomical localization of B cells. However, efficacious vaccines that rely on T cells for protection have been difficult to develop, as robust systemic memory T-cell responses do not necessarily correlate with host protection. In peripheral sites, tissue-resident memory T cells provide superior protection compared to circulating memory T cells. Here we describe a simple and non-inflammatory vaccine strategy that enables the establishment of a protective memory T-cell pool within peripheral tissue.

The female genital tract, which is a portal of entry for sexually transmitted infections, is an immunologically restrictive tissue that prevents entry of activated T cells in the absence of inflammation or infection. To overcome this obstacle, we developed a vaccine strategy that we term ‘prime and pull’ to establish local tissue-resident memory T cells at a site of potential viral exposure. This approach relies on two steps: conventional parenteral vaccination to elicit systemic T-cell responses (prime), followed by recruitment of activated T cells by means of topical chemokine application to the restrictive genital tract (pull), where such T cells establish a long-term niche and mediate protective immunity.

In mice, prime and pull protocol reduces the spread of infectious herpes simplex virus 2 into the sensory neurons and prevents development of clinical disease. These results reveal a promising vaccination strategy against herpes simplex virus 2, and potentially against other sexually transmitted infections such as human immunodeficiency virus.

A vaccine strategy that protects against genital herpes by establishing local memory T cells.
A vaccine strategy that protects against genital herpes by establishing local memory T cells.

Reactivation of genital herpes simplex virus type 2 infection in asymptomatic seropositive persons.

BACKGROUND Most persons who have serologic evidence of infection with herpes simplex virus (HSV) type 2 (HSV-2) are asymptomatic. Historically, it has been assumed that these persons have less frequent viral reactivation than those with symptomatic infection.METHODSWe conducted a prospective study to investigate genital shedding of HSV among 53 subjects who had antibodies to HSV-2 but who reported having no history of genital herpes, and we compared their patterns of viral shedding with those in a similar cohort of 90 subjects with symptomatic HSV-2 infection.

Genital secretions of the subjects in both groups were sampled daily and cultured for HSV for a median of 94 days.

RESULTS HSV was isolated from the genital mucosa in 38 of the 53 HSV-2-seropositive subjects (72 percent) who reported no history of genital herpes, and HSV DNA was detected by the polymerase-chain-reaction assay in cultures prepared from genital mucosal swabs in 6 additional subjects. The rate of subclinical shedding of HSV in the subjects with no reported history of genital herpes was similar to that in the subjects with such a history (3.0 percent vs. 2.7 percent).

Of the 53 subjects who had no reported history of genital herpes, 33 (62 percent) subsequently reported having typical herpetic lesions; the duration of their recurrences in these subjects was shorter (median, three days vs. five days; P<0.001) and the frequency lower (median, 3.0 per year vs. 8.2 per year; P<0.001) than in the 90 subjects with previously diagnosed symptomatic infection. Only 1 of these 53 subjects had no clinical or virologic evidence of HSV infection.

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EUR 524

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Bovine HGV IgG ELISA Kit

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E-10G 1 x 96 well plate
EUR 712
Description:

Bovine IgG ELISA Kit ; E-10G ; Immunology Consultants Laboratory

Species Reactivity: Bovine

Format: Sandwich ELISA

Product Type: ELISA Kit

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Bovine IgG ELISA Kit ; E-10GXSPP ; Immunology Consultants Laboratory

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EBP0665 96Tests
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OKIA00005-96W 1x96wellplate
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OKEH00513-96W 96Wells
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Bovine Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-b-48T 48T
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CSB-E12015B-24T 1 plate of 24 wells
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Description: Quantitative competitive ELISA kit for measuring Bovine Immunoglobulin G, IgG in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Immunoglobulin G, IgG ELISA Kit

1-CSB-E12015B
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative competitive ELISA kit for measuring Bovine Immunoglobulin G, IgG in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

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Bovine Phospholipid antibody (IgG)ELISA Kit

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ELISA kit for Bovine Tuberculosis IgG (TB-IgG)

KTE10466-48T 48T
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ELISA kit for Bovine Tuberculosis IgG (TB-IgG)

KTE10466-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Bovine Tuberculosis IgG (TB-IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Tuberculosis IgG (TB-IgG)

KTE10466-96T 96T
EUR 686.4
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Bovine IgG

31-AB20 50 mg
EUR 176
Description: Purified Bovine IgG

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QY-E60066 96T
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Bovine anti cardiolipin antibody IgG,ACA IgG ELISA kit

E01A79961 96T
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Bovine Brucella Antibody IgG, Brucella Ab IgG ELISA Kit

SL0240Bo - Ask for price

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SL0038Bo -
EUR 528

Bovine Japanese Encephalitis IgG, JEV IgG GENLISA ELISA

KLB0008 1 x 96 wells
EUR 341

IgG ELISA Kit (Bovine) : 96 Wells (OKEH00513)

OKEH00513 96 Wells
EUR 934.8
Description: Description of target: ;Species reactivity: Bovine;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.33 ng/mL

IgG ELISA Kit| Bovine Immunoglobulin G ELISA Kit

EF010968 96 Tests
EUR 826.8

Bovine Toxoplasma gondii IgG(Toxo-IgG) ELISA Kit

SL0199Bo - Ask for price

Bovine Leptospira antibody IgG (LEP-IgG) ELISA Kit

SL0121Bo - Ask for price

Bovine Leptospira antibody IgG (Leptospira AB IgG) ELISA Kit

YLA0363BO-48T 48T Ask for price

Bovine Leptospira antibody IgG (Leptospira AB IgG) ELISA Kit

YLA0363BO-96T 96T Ask for price

Bovine Immunoglobulin G ELISA Kit (IgG)

RK00471 96 Tests
EUR 625.2

Bovine Anti-sperm Anti-body IgG(AsAb IgG) ELISA Kit

NSL1946Bo 96 T
EUR 528

Bovine Brucella Anti-body (IgG) ELISA Kit

NSL2067Bo 96 T
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Bovine Japanese Encephalitis Antibody (IgG) (Bovine) ELISA Kit (OKCA00379)

OKCA00379 96 Wells
EUR 1131.6
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Bovine anti gliadin antibody (IgG) ELISA kit

E01A79978 96T
EUR 700
Description: ELISA

Bovine α Fodrin IgG/IgA ELISA Kit

NSL0020Bo 96 T
EUR 528

Bovine Rift Valley Fever IgG (RVF-IgG) ELISA Kit

SL0242Bo - Ask for price

ELISA kit for Bovine Paratuberculosis IgG (Para-TB IgG)

KTE10469-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Bovine Paratuberculosis IgG (Para-TB IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Paratuberculosis IgG (Para-TB IgG)

KTE10469-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Bovine Paratuberculosis IgG (Para-TB IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Paratuberculosis IgG (Para-TB IgG)

KTE10469-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Bovine Paratuberculosis IgG (Para-TB IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Bovine Thyroglobulin IgG Anti-body(TG IgG Ab) ELISA Kit

NSL1409Bo 96 T
EUR 528

Bovine Phosphatidylinositol antibody IgG ELISA kit

E01A85329 96T
EUR 700
Description: ELISA

Bovine A-Fodrin IgG/IgA ELISA Kit

EBA0756 96Tests
EUR 625.2

Bovine IgG Endotoxin Core Antibody ELISA kit

E01A83977 96T
EUR 700
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Bovine Mycobecterium tuberculosis Antibody (IgG) (Bovine) ELISA Kit (OKCA00381)

OKCA00381 96 Wells
EUR 1131.6
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Bovine Anti tissue transglutaminase IgG,IgG tTG ELISA kit

E01A80025 96T
EUR 700
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Bovine Anti myocardial antibody IgG ELISA kit

E01A78795 96T
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E01A78906 96T
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E01A80207 96T
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Bovine viral diarrhea virus antibody IgG(BVDV- IgG)ELISA Kit

SL0103Bo -
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Infectious bovine rhinotracheitis antibody IgG (IBR- IgG)ELISA Kit

SL0106Bo -
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Bovine IgG Fc receptor II (FCGR2) ELISA Kit

RK08382 96T
EUR 280

Bovine Toxoplasmosis (TOXO) Antibody (IgG) Elisa Kit

EK760814 96 Wells
EUR 0.93

Bovine IgG ELISA, No Crossreactivity with Mouse, Rat, Rabbit, or Human IgG

KT-623 96 tests
EUR 551

Bovine Anti-G2s Anti-body IgG(Anti-G2s IgG) ELISA Kit

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EUR 528

Bovine Anti-toxoplasma IgG Antibody (Anti-Toxo IgG) ELISA Kit

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Bovine β Anti-galactan proteins IgG(β AGP IgG) ELISA Kit

NSL0030Bo 96 T
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OKCA00379-96W - Bovine Japanese Encephalitis Antibody (IgG) (Bovine) ELISA Kit

OKCA00379-96W 96Wells
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Bovine Leptospira IgG antibody (Lep-Ab-IgG) ELISA Kit

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Description: Bovine (Bos taurus; Cattle)

Bovine Leptospira IgG antibody (Lep-Ab-IgG) ELISA Kit

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Description: Bovine (Bos taurus; Cattle)

Bovine anti Inhibin B antibody (IgG) ELISA kit

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Description: ELISA

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EUR 247
Description: Purified Bovine IgG (Fc)

Bovine Japanese Encephalitis Antibody(IgG)ELISA Kit

E01A84001 96T
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Description: ELISA

Bovine Japanese Encephalitis Antibody(IgG) Elisa Kit

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Bovine Anti-hepatitis E virus IgG (anti-HEV IgG)ELISA Kit

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Bovine Hp-IgG ELISA Kit

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EUR 625.2

Bovine IgG (HRP)

65C-CH0103 1 mg
EUR 169
Description: Purified Bovine IgG HRP conjugate

Bovine IgG (Fab)

31C-CH0110 2 mg
EUR 324
Description: Purified Bovine IgG (Fab)

Bovine β Anti galactan proteins IgG ELISA kit

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Description: ELISA

ELISA kit for Bovine Immunoglobulin G (IgG)

KTE10417-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Bovine Immunoglobulin G (IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Immunoglobulin G (IgG)

KTE10417-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Bovine Immunoglobulin G (IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Immunoglobulin G (IgG)

KTE10417-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Bovine Immunoglobulin G (IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Bovine Mycoplasma Pulmonis IgG Anti-body(MP IgG ab) ELISA Kit

NSL1025Bo 96 T
EUR 528

Bovine ACA-IgG ELISA Kit

EBA0705 96Tests
EUR 625.2

Bovine IgG (FITC)

65C-CH0102 1 mg
EUR 140
Description: Purified Bovine IgG FITC conjugate

Bovine anti-Hepatit E virus IgG(HEV-IgG) Elisa Kit

EK760658 96 Wells
EUR 0.93

Bovine Brucella IgG (Brucella-Ab) Elisa Kit

EK760017 96 Wells
EUR 0.89

Bovine Brucella IgG antibody (Brucella-Ab-IgG) ELISA Kit

AE62229BO-48Tests 48 Tests
EUR 360
Description: Bovine (Bos taurus; Cattle)

Bovine Brucella IgG antibody (Brucella-Ab-IgG) ELISA Kit

AE62229BO-96Tests 96 Tests
EUR 680
Description: Bovine (Bos taurus; Cattle)

Bovine Japanese Encephalitis Virus IgG (JEV-IgG)ELISA Kit

SL0063Bo -
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Bovine AnuA-IgG ELISA Kit

EBA0836 96Tests
EUR 625.2

Bovine Anti Integrin αVβ6 Antibody IgG ELISA kit

E01A80210 96T
EUR 700
Description: ELISA

Bovine β2 Glycoprotein 1 antibody IgG ELISA kit

E01A83171 96T
EUR 700
Description: ELISA

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E01A85627 96T
EUR 700
Description: ELISA

Bovine Anti-dsDNA IgG-specific ELISA Kit

NSL1910Bo 96 T
EUR 528

OKCA00381-96W - Bovine Mycobacterium tuberculosis Antibody (IgG) (Bovine) ELISA Kit

OKCA00381-96W 96Wells
EUR 775

Bovine Anti-Transglutaminase Antibodies IgG ELISA kit

E01A80199 96T
EUR 700
Description: ELISA

ELISpot Flex: Bovine IgG (ALP)

3150-2A for 4 plates
EUR 498.75

ELISpot Flex: Bovine IgG (HRP)

3150-2H for 4 plates
EUR 498.75

Bovine anti Endomysial antibody (EMA)(IgG) ELISA kit

E01A79772 96T
EUR 700
Description: ELISA

Bovine mycobecterium tuberculosis antibody(IgG) Elisa Kit

EK760272 96 Wells
EUR 0.92

Bovine mycobecterium tuberculosis antibody(IgG) ELISA Kit

CSB-E17362B-24T 1 plate of 24 wells
EUR 198
Description: Qualitativeindirect ELISA kit for measuring Bovine mycobecterium tuberculosis antibody (IgG) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine mycobecterium tuberculosis antibody(IgG) ELISA Kit

1-CSB-E17362B
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Qualitativeindirect ELISA kit for measuring Bovine mycobecterium tuberculosis antibody(IgG) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

CONCLUSION SSeropositivity for HSV-2 is associated with viral shedding in the genital tract, even in subjects with no reported history of genital herpes.