Therapeutic HSV-2 vaccine decreases recurrent virus shedding and recurrent genital herpes disease.

Therapeutic HSV-2 vaccine decreases recurrent virus shedding and recurrent genital herpes disease.

Genital herpes simplex virus (HSV) sort 2 is a standard persistent an infection that ceaselessly reactivates to trigger recurrent lesions and recurrent viral shedding which is incompletely managed by antiviral remedy. GEN-003 is a candidate therapeutic vaccine containing 2 HSV-2 proteins, gD2 and ICP4, and Matrix-M2 adjuvant (M2).HSV-2 seropositive individuals with genital herpes have been randomized into three dose cohorts of Gen-003 (60 µg antigen/50 µg M2, 60 µg/75 µg M2 or Placebo). Three intramuscular doses 21 days aside of GEN-003 or placebo have been administered. Participants obtained genital space swabs twice-daily for HSV-2 detection and monitored genital lesions for 12 months.

 

The charges of virus shedding and lesion charges earlier than vaccination have been in comparison with three outlined durations after vaccination; Days 43-71, Month 6 and Month 12.GEN-003 at a dose of 60 µg every antigen/50 µg M2 decreased HSV shedding instantly after dosing with a fee ratio of 0.58, in comparison with 0.75 for the GEN-003 60 µg/75 µg M2 and 1.06 for placebo. Lesion charges, recurrence charges, and period of recurrences have been additionally decreased. Reactogenicity was greater with the 75 µg M2 dose than the 50 µg M2 dose, particularly for ache, tenderness, malaise and fatigue. Antibody and mobile immune responses have been stimulated by each doses and continued to 12 months.GEN-003 vaccine manufactured with a scalable course of gave outcomes much like these noticed in prior medical trials. GEN-003 had an appropriate security profile and stimulated each humoral and mobile immune responses. The 60 µg antigen/50 µg M2 supplied the maximal impact on virologic and medical measures and warrants additional improvement. (Funded by Genocea; ClinicalTrials.gov quantity.

 

 

Duration of safety from stay attenuated vs. sub unit HSV-2 vaccines within the guinea pig mannequin of genital herpes: Reassessing efficacy utilizing endpoints from medical trials.

Although herpes simplex viruses (HSV) are a serious goal for vaccine improvement no vaccine is presently licensed.A stay attenuated HSV virus vaccine, VC2 was in comparison with a subunit HSV vaccine, glycoprotein D (gD2) administered with the adjuvant, MPL/Alum utilizing the guinea pig mannequin of genital herpes. Three doses of intramuscular (IM) vaccine have been supplied adopted by intravaginal problem with HSV-2 at both three weeks or six months after the final vaccination.Both VC2 and gD2 vaccines decreased acute genital illness. VC2 was considerably more practical in lowering acute vaginal replication, the quantity of virus in neural tissue, subsequent recurrent illness and recurrent virus shedding following problem at three weeks submit vaccination.
Both vaccines continued to offer safety at 6 months after vaccination however the variations between the vaccines grew to become extra pronounced in favor of the stay attenuated vaccine, VC2. Significant variations in acute illness, acute vaginal virus replication, recurrent illness and recurrent virus shedding (P<0.05 for every) was noticed evaluating the vaccines. Re-examination of safety for this examine utilizing standards much like these utilized in current medical trials (inclusion of recurrent illness) confirmed that efficacy might not be as excessive on this mannequin as beforehand thought prompting a must assess the most effective predictive outcomes for defense in people.
While each the stay attenuated vaccine, VC2, and the gD2 subunit vaccine supplied safety, the period of safety seemed to be better for VC2. Using the identical analysis standards as utilized in human trials supplied distinctive insights into the utility of the guinea pig mannequin. After utility to recurrent lesions, native erythema, edema, scabbing and/or flaking, ache, burning, and maximal lesion measurement have been considerably better within the imiquimod group than within the car group. The examine was terminated early due to extreme native adversarial occasions that occurred in 5 recipients of imiquimod. The median time till the subsequent recurrence was, nonetheless, elevated from 50 days within the car group to 91 days within the imiquimod group.
 Therapeutic HSV-2 vaccine decreases recurrent virus shedding and recurrent genital herpes disease.
Therapeutic HSV-2 vaccine decreases recurrent virus shedding and recurrent genital herpes disease.

Valacyclovir prophylaxis to stop recurrent herpes at supply: a randomized medical trial.

OBJECTIVE
To measure the efficacy of valacyclovir suppression in late being pregnant to scale back the incidence of recurrent genital herpes in labor and subsequent cesarean supply.
METHODS
A complete of 350 pregnant ladies with a historical past of genital herpes have been assigned randomly to oral valacyclovir 500 mg twice a day or an an identical placebo from 36 weeks of gestation till supply. In labor, vulvovaginal herpes simplex virus (HSV) tradition and polymerase chain response (PCR) specimens have been collected. Vaginal supply was permitted if no medical recurrence or prodromal signs have been current. Neonatal HSV cultures and laboratory exams have been obtained, and infants have been adopted up for 1 month after supply. Data have been analyzed utilizing chi2 and Student t exams.

Green qPCR SuperMix UDG

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Top Green qPCR SuperMix

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Library Quantification qPCR SuperMix

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Pro QPCR SuperMix Kit - ROX premixed

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cDNA Synthesis SuperMix for qPCR

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QCell-Eva One-Step qRT-PCR SuperMix Kit

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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

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PCR SuperMix

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OneScriptcDNA Synthesis SuperMix

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Evo? cDNA Supermix

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PCR SuperMix (-dye)

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qPCR Control Template Kit

G929 100 rxns
EUR 148

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

QPCR Positive Control Kit

M1126-100
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OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
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G454 100 x 20 ul reactions
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T PCR SuperMix (-dye)

20-abx098005
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EasyPfu PCR SuperMix (-dye)

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FastPfu PCR SuperMix (-dye)

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Methylamp MS-qPCR Fast Kit

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Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
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PCR SuperMix for PAGE (+dye)

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Frit Kit

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LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
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Column Packing Kit

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PCR Mycoplasma Detection Kit

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Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
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QCell-Pro One-Step qRT-PCR SuperMix Kit

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QCell-Pro One-Step qRT-PCR SuperMix Kit

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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

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Coronavirus Rapid RT-qPCR Detection Kit

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Adeno-Associated Virus qPCR Quantification Kit

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igScript RT-qPCR Kit - 50 Reactions

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All-in-One cDNA Synthesis SuperMix

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EUR 456
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miRNA First-Strand cDNA Synthesis SuperMix

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Green Two-Step qRT-PCR SuperMix

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Green One-Step qRT-PCR SuperMix

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Probe One-Step qRT-PCR SuperMix

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Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
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Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
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CASLV225PA-KIT 1 Kit
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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

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Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

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Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 331

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

First-Strand cDNA Synthesis SuperMix for PCR

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EUR 578

Two-Step RT-PCR SuperMix (12 kb)

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Two-Step RT-PCR SuperMix (15 kb)

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Green miRNA Two-Step qRT-PCR SuperMix

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PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
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BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

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EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

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EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

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EUR 213

BrightGreen 5X qPCR MasterMix-ROX

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EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 646

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-520 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-522 10 x 1,25 ml
EUR 1719

igScript One Step RT-qPCR Kit - 100 Reactions

4214 1/EA
EUR 253

igScript One Step RT-qPCR Kit - 500 Reactions

4218 1/EA
EUR 773

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 217.00
  • EUR 356.00
  • 1 ml
  • 5 ml

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 606

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 662

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 857

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 801
RESULTS
One hundred seventy ladies handled with valacyclovir and 168 ladies handled with placebo have been evaluated. Eighty-two % of the ladies had recurrent genital herpes; 12% had first episode, nonprimary genital herpes; and 6% had first episode, main genital herpes. At supply, 28 ladies (8%) had recurrent genital herpes requiring cesarean supply: 4% within the valacyclovir group and 13% within the placebo group (P = .009). Herpes simplex virus was detected by tradition in 2% of the valacyclovir group and 9% [corrected] of the placebo group (P =.02). No infants have been recognized with neonatal HSV, and there have been no vital variations in neonatal issues. There have been no vital variations in maternal or obstetric issues in both group.

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